Characteristics of Three Laccase Isoforms from the Basidiomycete Pleurotus nebrodensis
نویسندگان
چکیده
Figure S1. Cont. 68 PnLac1 95 PoLac 95 PsLac 95 PpLac 95 LsLac 65 HmLac . M M M M . . V V V V . . L L L L . . S S S S . . T T T T . . K K K K . . L L L L . . A A V V . . A A A A . . L L L L . . V V V V . . A A A A . . S S S S . . L L L L . . P P P P . . F F F F . . V V V V . . L L F F . . A A A A . . A A V V . . T T T T . . K K K K . . K K K K . . L L L L . . D D D D . . F F F F . . H H H H . . I I I I . R R R R R G N N N N N F D D D D D F V V V V V Q V V V V V A S S S S S R P P P P P T D D D D D S G G G G G G F F F F F M E E E E E D R R R R R G R . . . . P R . . . . . R R R R R S A A A A A F I I I I I V T T T T T N V V V V V Q N N N N N C G G G G G P I I I I I I F F F F F P P P P P P P G G G G G N T T T T T T P P P P P T V V V V V F I I I I I L L L L L L Y Q Q Q Q Q D K K K K K F N N N N N D K . . . . . N . . . . . D D D D D T K K K K K A V V V V V G Q Q Q Q Q Q I I I I I T N N N K K G T T T T T N I I I I I F N N N N N W E E E E E Y L L L L L H T T T T T S D D D D D H P P P P P L G G G G G S M M M M M T R R R R R Q R R R R R Y R . . . . C S S S S S D T T T T T G S S S S S L I I I I I R H H H H H G W W W W W V H H H H H F G G G G G P L L L L L V F F F F F Y Q Q Q Q Q D H H H H H P K K K K K K T T T T T D . S S S S . . G G G G . . M M M M . . D D D D .
منابع مشابه
Biochemical Characteristics of Three Laccase Isoforms from the Basidiomycete Pleurotus nebrodensis.
The characterization of three laccase isoforms from Pleurotus nebrodensis is described. Isoenzymes Lac1, Lac2 and Lac3 were purified to homogeneity using ion exchange chromatography on DEAE-cellulose, CM-cellulose and Q-Sepharose and a gel filtration step on Superdex 75. The molecular weights of the purified laccases were estimated to be 68, 64 and 51 kDa, respectively. The isoenzymes demonstra...
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Three laccase isoenzymes (Lac1, Lac2 and Lac3) have been purified to homogeneity from Pleurotus nebrodensis in our previous study. Lac2 was shown to be the dominant isoform, capable of oxidizing the majority of laccase substrates and manifesting good thermostability and pH stability. Hence, Lac2 was selected to decolourize structurally different dyes and the colour removal efficiencies of Lac2 ...
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